| 1 |  Xavier Tondo Volpini (ESP) | Movistar | 17:01:40 |
| 2 |  Bauke Mollema (P-B) | Rabobank | 0:00:09 |
| 3 | Igor Antón Hernandez (ESP) | Euskaltel - Euskadi | 0:00:17 |
| 4 |  Domenico Pozzovivo (ITA) | Colnago - CSF Inox | 0:00:40 |
| 5 |  Jérôme Coppel (FRA) | Saur - Sojasun | 0:00:47 |
| 6 |  Juan Pablo Suarez Suarez (COL) | Une - Epm | mt |
| 7 | Dario David Cioni (ITA) | Team Ineos | 0:00:54 |
| 8 | Ricardo Garcia Ambroa (ESP) | Orbea | 0:00:58 |
| 9 | Juan Manuel Gárate Cepa (ESP) | Rabobank | 0:00:59 |
| 10 | Laurens Ten Dam (P-B) | Rabobank | 0:01:16 |
| 11 | Santiago Pérez Fernández (ESP) | Barbot - Efapel | mt |
| 12 | Robinson Eduardo Chalapud Gomez (COL) | Colombia Es Passion - Cafe de Colombia | 0:01:21 |
| 13 | Steven Kruijswijk (P-B) | Rabobank | 0:01:22 |
| 14 |  Chris Froome (G-B) | Team Ineos | 0:01:32 |
| 15 | Nairo Alexander Quintana Rojas (COL) | Colombia Es Passion - Cafe de Colombia | 0:01:42 |
| 16 | Giovanny Manuel Baez Alvarez (COL) | Une - Epm | mt |
| 17 | Sergio Pardilla Bellón (ESP) | Movistar | 0:01:44 |
| 18 | John Edilberto Martinez Montero (COL) | Colombia Es Passion - Cafe de Colombia | 0:01:45 |
| 19 | John Darwin Atapuma Hurtado (COL) | Colombia Es Passion - Cafe de Colombia | 0:01:46 |
| 20 | Fabrice Jeandesboz (FRA) | Saur - Sojasun | 0:02:00 |
| 21 | Giampaolo Cheula (ITA) | Geox - TMC | 0:02:27 |
| 22 | Mikel Nieve Iturralde (ESP) | Euskaltel - Euskadi | 0:02:42 |
| 23 | Filippo Savini (ITA) | Colnago - CSF Inox | 0:02:47 |
| 24 | Carlos Fernando Piamonte Rodriguez (COL) | Une - Epm | 0:03:21 |
| 25 | Tom Jelte Slagter (P-B) | Rabobank | 0:03:42 |
| 26 | Víctor De La Parte Gonzalez (ESP) | Caja Rural | 0:04:13 |
| 27 | Jesús Hernández Blázquez (ESP) | Saxo Bank - Sungard | 0:04:18 |
| 28 | Adrian Palomares Villaplana (ESP) | Andalucia - Caja Granada | 0:04:43 |
| 29 |  Sérgio Ferreira Sousa (POR) | Barbot - Efapel | 0:04:54 |
| 30 | Hector Leonardo Paez Leon (COL) | Colombia Es Passion - Cafe de Colombia | 0:05:03 |
| 31 | Francisco Javier Aramendia Llorente (ESP) | Euskaltel - Euskadi | 0:05:14 |
| 32 | Pablo Lastras Garcia (ESP) | Movistar | 0:05:15 |
| 33 | Stefano Pirazzi (ITA) | Colnago - CSF Inox | 0:05:25 |
| 34 | Walter Fernando Pedraza Morales (COL) | Une - Epm | 0:05:41 |
| 35 | Víctor Cabedo Carda (ESP) | Orbea | 0:07:02 |
| 36 | Rafael Infantino Abreu (COL) | Une - Epm | 0:07:57 |
| 37 | Sergio Miguel Vieira Ribeiro (POR) | Barbot - Efapel | 0:08:46 |
| 38 | Mikel Bizkarra Etxeguibel (ESP) | Orbea | 0:09:44 |
| 39 | Victor Hugo Peña Grisales (COL) | Colombia Es Passion - Cafe de Colombia | 0:10:04 |
| 40 | Alex Alberto Cano Ardila (COL) | Colombia Es Passion - Cafe de Colombia | 0:10:19 |
| 41 | Camilo Andres Suarez Albarracin (COL) | Colombia Es Passion - Cafe de Colombia | 0:10:33 |
| 42 | José Alberto Benitez Roman (ESP) | Andalucia - Caja Granada | 0:10:35 |
| 43 | Egoitz Garcia Echeguibel (ESP) | Caja Rural | 0:11:59 |
| 44 | Russell Downing (G-B) | Team Ineos | 0:12:00 |
| 45 | Federico Canuti (ITA) | Colnago - CSF Inox | 0:12:09 |
| 46 | Ruben Perez Moreno (ESP) | Euskaltel - Euskadi | 0:12:13 |
| 47 | Ludovic Turpin (FRA) | Saur - Sojasun | 0:12:15 |
| 48 |  Carlos Ivan Oyarzun Guiñez (CHI) | Movistar | 0:12:20 |
| 49 | Carlos Verona Quintanilla (ESP) | Burgos 2016 - Castilla y Leon | 0:12:28 |
| 50 | David De La Cruz Melgarejo (ESP) | Caja Rural | mt |
| 51 | Carlos Sastre Candil (ESP) | Geox - TMC | 0:12:29 |
| 52 | Peter Kennaugh (G-B) | Team Ineos | 0:12:44 |
| 53 |  Fabricio Ferrari Barcelo (URU) | Caja Rural | 0:12:46 |
| 54 |  Matthias Brändle (AUT) | Geox - TMC | 0:13:09 |
| 55 |  Jacques Janse Van Rensburg (AFS) | Burgos 2016 - Castilla y Leon | 0:13:11 |
| 56 | Jose Luis Roldan Carmona (ESP) | Andalucia - Caja Granada | 0:13:45 |
| 57 |  Richie Porte (AUS) | Saxo Bank - Sungard | 0:13:47 |
| 58 | Diego Gallego Arnaiz (ESP) | Burgos 2016 - Castilla y Leon | 0:13:51 |
| 59 | Daniel Navarro García (ESP) | Saxo Bank - Sungard | 0:13:53 |
| 60 | Yannick Talabardon (FRA) | Saur - Sojasun | 0:13:59 |
| 61 | Jorge Azanza Soto (ESP) | Euskaltel - Euskadi | 0:14:11 |
| 62 | Ben Swift (G-B) | Team Ineos | 0:16:07 |
| 63 | Francisco José Ventoso Alberdi (ESP) | Movistar | 0:16:45 |
| 64 |  Serge Pauwels (BEL) | Team Ineos | 0:16:56 |
| 65 | Guillaume Levarlet (FRA) | Saur - Sojasun | 0:17:06 |
| 66 | Filipe Duarte Sousa Cardoso (POR) | Barbot - Efapel | 0:17:18 |
| 67 | Rui Miguel Sousa Barbosa (POR) | Barbot - Efapel | 0:17:33 |
| 68 | Raul Alarcon Garcia (ESP) | Barbot - Efapel | 0:17:56 |
| 69 | Jaime Alberto Castañeda Ortega (COL) | Une - Epm | 0:17:58 |
| 70 | Francisco Javier Iriarte Garro (ESP) | Movistar | 0:18:34 |
| 71 | Alex Dowsett (G-B) | Team Ineos | 0:18:49 |
| 72 |  Branislau Samoilau (BLR) | Movistar | 0:19:51 |
| 73 | Juan Mauricio Soler Hernandez (COL) | Movistar | 0:20:11 |
| 74 | Jonathan Hivert (FRA) | Saur - Sojasun | 0:20:45 |
| 75 | Simone Stortoni (ITA) | Colnago - CSF Inox | 0:24:28 |
| 76 | Manuel Belletti (ITA) | Colnago - CSF Inox | 0:24:31 |
| 77 | Juan José Lobato del Valle (ESP) | Andalucia - Caja Granada | 0:24:42 |
| 78 |  Marcel Wyss (SUI) | Geox - TMC | mt |
| 79 | Antonio Piedra Perez (ESP) | Andalucia - Caja Granada | 0:24:47 |
| 80 | Luke Roberts (AUS) | Saxo Bank - Sungard | 0:24:58 |
| 81 | Pascual Orengo Lopez (ESP) | Burgos 2016 - Castilla y Leon | 0:25:05 |
| 82 | Pierre Cazaux (FRA) | Euskaltel - Euskadi | 0:25:08 |
| 83 | Antonio Felipe Amorim Oliveira (POR) | Barbot - Efapel | 0:25:12 |
| 84 | Joaquin Sobrino Martinez (ESP) | Caja Rural | mt |
| 85 | Diego Milán Jiménez (ESP) | Caja Rural | 0:25:20 |
| 86 | Noel Martín Infante (ESP) | Orbea | 0:25:27 |
| 87 | Lluís Guillermo Mas Bonet (ESP) | Burgos 2016 - Castilla y Leon | 0:25:46 |
| 88 |  Volodymir Gustov (UKR) | Saxo Bank - Sungard | 0:26:12 |
| 89 | Jon Aberasturi Izaga (ESP) | Orbea | 0:26:17 |
| 90 | Francisco Manuel Anton Marin (ESP) | Burgos 2016 - Castilla y Leon | 0:26:19 |
| 91 | Vicente David Bernabéu Armengol (ESP) | Andalucia - Caja Granada | 0:26:33 |
| 92 | Gianluca Brambilla (ITA) | Colnago - CSF Inox | 0:26:35 |
| 93 | Antonio Cabello Baena (ESP) | Andalucia - Caja Granada | 0:26:50 |
| 94 | Ruben Martinez Hernandez (ESP) | Caja Rural | 0:26:58 |
| 95 | Javier Ramirez Abeja (ESP) | Andalucia - Caja Granada | 0:27:34 |
| 96 | Benjamin Noval Gonzalez (ESP) | Saxo Bank - Sungard | 0:28:11 |
| 97 | Paolo Locatelli (ITA) | Colnago - CSF Inox | 0:28:43 |
| 98 | David Gutierrez Gutierrez (ESP) | Geox - TMC | 0:29:06 |
| 99 | Aritz Etxebarria Etxebarria (ESP) | Orbea | 0:29:21 |
| 100 | Julien Simon (FRA) | Saur - Sojasun | 0:29:25 |
| 101 | Theo Bos (P-B) | Rabobank | 0:29:27 |
| 102 | Matteo Pelucchi (ITA) | Geox - TMC | 0:30:01 |
| 103 | Daniel Sesma Sorbet (ESP) | Euskaltel - Euskadi | 0:30:27 |
| 104 | Carlos Baltazar (POR) | Barbot - Efapel | 0:31:08 |
| 105 | Ruben Jimenez Ventosa (ESP) | Burgos 2016 - Castilla y Leon | 0:32:11 |
| 106 | Graeme Allen Brown (AUS) | Rabobank | 0:32:43 |
