| 1 |  Franziska Brauße (ALL) | Ceratizit - Wnt | 0:02:56 |
| 2 |  Fien Van Eynde (BEL) | Fenix-Deceuninck Development Team | 0:01 |
| 3 |  Katarzyna Wilkos (POL) | | mt |
| 4 |  Marion Norbert Riberolle (FRA) | Fenix-Deceuninck Development Team | 0:02 |
| 5 |  Valeriya Kononenko (UKR) | | 0:04 |
| 6 | Patrycja Lorkowska (POL) | | mt |
| 7 |  Aniek van Alphen (P-B) | Fenix-Deceuninck Development Team | mt |
| 8 |  Nikola Bajgerová (RTC) | | 0:05 |
| 9 |  Alessia Vigilia (ITA) | | 0:06 |
| 10 | Giorgia Bariani (ITA) | | mt |
| 11 | Ceylin del Carmen Alvarado (P-B) | Fenix-Deceuninck | mt |
| 12 | Eliska Kvasnicková (RTC) | | mt |
| 13 | Arianna Fidanza (ITA) | Ceratizit - Wnt | 0:08 |
| 14 | Anne Knijnenburg (P-B) | | mt |
| 15 |  Fabienne Buri (SUI) | | mt |
| 16 | Anaïs Morichon (FRA) | Arkéa Pro Cycling Team | mt |
| 17 | Natalia Krzeslak (POL) | | 0:09 |
| 18 | Lucie Liboreau (FRA) | Arkéa Pro Cycling Team | mt |
| 19 |  Megan Armitage (IRL) | Arkéa Pro Cycling Team | 0:10 |
| 20 |  Anastasiya Kolesava (BLR) | Arkéa Pro Cycling Team | mt |
| 21 | Alice Palazzi (ITA) | | mt |
| 22 | Carlotta Cipressi (ITA) | | mt |
| 23 | Sara van De Vel (BEL) | Fenix-Deceuninck Development Team | 0:11 |
| 24 | Monika Brzezna (POL) | | mt |
| 25 | Yuliia Biriukova (UKR) | | mt |
| 26 | Femke Gerritse (P-B) | Parkhotel Valkenburg | mt |
| 27 |  Valeria Valgonen (RUS) | Massi - Tactic | mt |
| 28 |  Miryan Maritza Nuñez Padilla (EQU) | Massi - Tactic | mt |
| 29 | Alice Maria Arzuffi (ITA) | Ceratizit - Wnt | mt |
| 30 | Maaike Coljé (P-B) | Arkéa Pro Cycling Team | mt |
| 31 | Nikol Plosaj (POL) | | mt |
| 32 | Silvia Magri (ITA) | Team Jayco Alula | 0:12 |
| 33 | Wiktoria Pikulik (POL) | | mt |
| 34 | Zuzanna Chylinska (POL) | | mt |
| 35 | Lana Eberle (ALL) | Ceratizit - Wnt | mt |
| 36 | Imogen Cotter (IRL) | Fenix-Deceuninck Development Team | mt |
| 37 | Lea Fuchs (SUI) | Born to Win G20 Ambedo | 0:13 |
| 38 | Karoline Goldschmidt (ALL) | | mt |
| 39 | Kirstie van Haaften (P-B) | Parkhotel Valkenburg | 0:14 |
| 40 | Anastasia Carbonari (ITA) | | mt |
| 41 | Beatrice Caudera (ITA) | | mt |
| 42 | Nikola Nosková (RTC) | | mt |
| 43 | Matilde Vitillo (ITA) | Be Pink | mt |
| 44 |  Agua Marina Espínola Salinas (PAR) | | 0:15 |
| 45 | Greta Richioud (FRA) | | mt |
| 46 | Olha Kulynych (UKR) | Duolar - Chevalmeire | mt |
| 47 | Nele Laing (ALL) | | mt |
| 48 | Francesca Baroni (ITA) | | mt |
| 49 |  Ashley Frye (E-U) | | mt |
| 50 | Quinty Schoens (P-B) | Parkhotel Valkenburg | mt |
| 51 | Elisa De Vallier (ITA) | | 0:16 |
| 52 | Milena Del Sarto (ITA) | Aromitalia - Basso Bikes - Vaiano | mt |
| 53 | Katharina Becker (ALL) | | mt |
| 54 | Cara O'Neill (E-U) | | mt |
| 55 | Claire Windsor (E-U) | | mt |
| 56 | Valeria Curnis (ITA) | | mt |
| 57 | Marta Pavesi (ITA) | | mt |
| 58 | Asia Zontone (ITA) | Isolmant - Premac - Vittoria | mt |
| 59 |  Rasa Leleivyte (LTU) | Aromitalia - Basso Bikes - Vaiano | mt |
| 60 | Giulia Giuliani (ITA) | GB Junior Team Piemonte Pedale Castanese A.S.D. | 0:17 |
| 61 |  Hanna Nilsson (SUE) | Ceratizit - Wnt | mt |
| 62 |  Ainhoa Moreno Ossowsky (ESP) | Aromitalia - Basso Bikes - Vaiano | mt |
| 63 |  Safia Al Sayegh (EAU) | Uae Team Adq | mt |
| 64 | Laura Asencio (FRA) | | mt |
| 65 | Barbora Nemcová (RTC) | | mt |
| 66 | Gemma Sernissi (ITA) | GB Junior Team Piemonte Pedale Castanese A.S.D. | mt |
| 67 | Giulia Vallotto (ITA) | | mt |
| 68 | Katerina Kohoutková (RTC) | | mt |
| 69 | Gabriela Bártová (RTC) | | 0:18 |
| 70 | Francesca Tommasi (ITA) | | mt |
| 71 | Victoire Joncheray (FRA) | | mt |
| 72 | Tetiana Yashchenko (UKR) | | mt |
| 73 | Carmela Cipriani (ITA) | Isolmant - Premac - Vittoria | mt |
| 74 | Janine Schneider (ALL) | | mt |
| 75 | Noemi Lucrezia Eremita (ITA) | Isolmant - Premac - Vittoria | mt |
| 76 | Angela Oro (ITA) | | mt |
| 77 | Sara Pepoli (ITA) | Isolmant - Premac - Vittoria | mt |
| 78 |  Jennifer Tatiana Ducuara Acevedo (COL) | Massi - Tactic | mt |
| 79 | Emma Redaelli (ITA) | | mt |
| 80 | Maja Tracka (POL) | | mt |
| 81 | Gaia Segato (ITA) | | 0:18 |
| 82 | Michela De Grandis (ITA) | | 0:19 |
| 83 | Valentina Basilico (ITA) | Be Pink | mt |
| 84 | Elena Contarin (ITA) | | mt |
| 85 | Nela Slaníková (RTC) | | mt |
| 86 | Sonia Rossetti (ITA) | GB Junior Team Piemonte Pedale Castanese A.S.D. | mt |
| 87 | Elisa Valtulini (ITA) | | mt |
| 88 | Chiara Reghini (ITA) | | mt |
| 89 | Sara Casasola (ITA) | Born to Win G20 Ambedo | 0:20 |
| 90 |  Nesrine Houili (ALG) | | mt |
| 91 | Beatrice Rossato (ITA) | Isolmant - Premac - Vittoria | mt |
| 92 | Dorota Przezak (POL) | | mt |
| 93 | Yana Myalitsina (RUS) | | mt |
| 94 | Ségolène Thomas (FRA) | | mt |
| 95 | Alessia Foligno (ITA) | | mt |
| 96 | Kayla Davis (E-U) | Israel Premier Tech Roland | 0:21 |
| 97 |  Anabel María Fernanda Yapura Plaza (ARG) | | mt |
| 98 | Patricia Ortega Ruiz (ESP) | Massi - Tactic | mt |
| 99 | Lara Röhricht (ALL) | | mt |
| 100 | Julia van Bokhoven (P-B) | Parkhotel Valkenburg | mt |
| 101 |  Diane Ingabire (RWA) | | 0:22 |
| 102 | Jadee Treffeisen (ALL) | | mt |
| 103 | Beatrice Pozzobon (ITA) | | mt |
| 104 | Prisca Savi (ITA) | Be Pink | mt |
| 105 | Alice Capasso (ITA) | | mt |
| 106 | Sara Luccon (ITA) | | mt |
| 107 | Nicole Hartychová (RTC) | | mt |
| 108 | Lara Scarselli (ITA) | Aromitalia - Basso Bikes - Vaiano | mt |
| 109 |  Adar Shriki (ISR) | Israel Premier Tech Roland | mt |
| 110 | Sara Piffer (ITA) | | mt |
| 111 | Annika Schelb (ALL) | | 0:23 |
| 112 | Martina Testa (ITA) | | mt |
| 113 | Lucy Mayrhofer (ALL) | | mt |
| 114 | Martina Silvestri (ITA) | | mt |
| 115 | Nofar Maoz (ISR) | Israel Premier Tech Roland | mt |
| 116 | Yelyzaveta Holod (UKR) | | mt |
| 117 | Anna Hicks (E-U) | | mt |
| 118 | Romina Costantini (ITA) | | 0:24 |
| 119 | Marta Romance I Oms (ESP) | | mt |
| 120 |  Emilie Fortin (CAN) | | 0:25 |
| 121 | Marie Lagershausen (ALL) | | mt |
| 122 |  Daniela Schmidsberger (AUT) | | 0:26 |
| 123 | Monica Castagna (ITA) | | mt |
| 124 | Giulia Miotto (ITA) | GB Junior Team Piemonte Pedale Castanese A.S.D. | mt |
| 125 | Nika Myalitsina (RUS) | | mt |
| 126 | Maayan Tzur (ISR) | Israel Premier Tech Roland | 0:27 |
| 127 | Marina Varenyk (UKR) | Eneicat - Cmteam - Seguros Deportivos | 0:28 |
| 128 |  Eyeru Tesfoam Gebru (ETH) | | mt |
| 129 | Virginia Bortoli (ITA) | | mt |
| 130 |  Tess Lawson (G-B) | | mt |
| 131 | Nataliia Sofroniuk (UKR) | | mt |
| 132 | Alina Bohdan (UKR) | | 0:29 |
| 133 | Beatrice Roda (ITA) | | 0:30 |
| 134 | Fabia Maramotti (ITA) | | mt |
| 135 | Kamila Pastuszek (POL) | | mt |
| 136 | Viktoriia Parkhomiuk (UKR) | | mt |
| 137 |  Evelina Ermane-Marcenko (LAT) | | mt |
| 138 |  Llori Sharpe (JAM) | | 0:31 |
| 139 | Kitija Siltumena (LAT) | | mt |
| 140 | Valantine Nzayisenga (RWA) | | 0:32 |
| 141 | Tatiana Chili (ITA) | | mt |
| 142 | Anastasiia Ishman (UKR) | | mt |
| 143 | Laura Belohvoscika (LAT) | | 0:34 |
| 144 | Iris Gómez Argiles (ESP) | | 0:35 |
| 145 | Lasma Elza Vaivode (LAT) | | mt |
| 146 | Anja Bertleff (ALL) | | 0:38 |
| 147 | Esther Luna Gazzola (ITA) | | 0:39 |
| 148 | Vittoria Ruffilli (ITA) | GB Junior Team Piemonte Pedale Castanese A.S.D. | mt |
| 149 |  Sara Nicole Torrico Ortiz (BOL) | | |
| 150 | Laura Sofia Castillo Murillo (COL) | | |
