| 1 |  Rasa Leleivyte (LTU) | Aromitalia - Basso Bikes - Vaiano | 2:27:28 |
| 2 |  Alessia Vigilia (ITA) | | mt |
| 3 | Silvia Magri (ITA) | Team Jayco Alula | mt |
| 4 |  Greta Richioud (FRA) | | mt |
| 5 | Elisa De Vallier (ITA) | | mt |
| 6 |  Megan Armitage (IRL) | Arkéa Pro Cycling Team | mt |
| 7 | Anastasia Carbonari (ITA) | | 0:04 |
| 8 |  Anastasiya Kolesava (BLR) | Arkéa Pro Cycling Team | mt |
| 9 |  Katarzyna Wilkos (POL) | | mt |
| 10 | Angela Oro (ITA) | | mt |
| 11 | Natalia Krzeslak (POL) | | mt |
| 12 | Michela De Grandis (ITA) | | mt |
| 13 |  Hanna Nilsson (SUE) | Ceratizit - Wnt | mt |
| 14 |  Olha Kulynych (UKR) | Duolar - Chevalmeire | mt |
| 15 | Arianna Fidanza (ITA) | Ceratizit - Wnt | mt |
| 16 |  Ceylin del Carmen Alvarado (P-B) | Fenix-Deceuninck | mt |
| 17 |  Yana Myalitsina (RUS) | | mt |
| 18 |  Miryan Maritza Nuñez Padilla (EQU) | Massi - Tactic | mt |
| 19 | Prisca Savi (ITA) | Be Pink | 0:10 |
| 20 |  Ainhoa Moreno Ossowsky (ESP) | Aromitalia - Basso Bikes - Vaiano | mt |
| 21 | Laura Asencio (FRA) | | mt |
| 22 | Patrycja Lorkowska (POL) | | mt |
| 23 | Elisa Valtulini (ITA) | | 0:14 |
| 24 | Aniek van Alphen (P-B) | Fenix-Deceuninck Development Team | 0:16 |
| 25 | Carlotta Cipressi (ITA) | | 0:18 |
| 26 | Asia Zontone (ITA) | Isolmant - Premac - Vittoria | 0:21 |
| 27 |  Eyeru Tesfoam Gebru (ETH) | | mt |
| 28 | Alice Maria Arzuffi (ITA) | Ceratizit - Wnt | mt |
| 29 |  Agua Marina Espínola Salinas (PAR) | | 0:24 |
| 30 | Valeria Valgonen (RUS) | Massi - Tactic | mt |
| 31 |  Nikola Nosková (RTC) | | mt |
| 32 | Quinty Schoens (P-B) | Parkhotel Valkenburg | mt |
| 33 |  Nele Laing (ALL) | | mt |
| 34 |  Lea Fuchs (SUI) | Born to Win G20 Ambedo | 0:28 |
| 35 | Valentina Basilico (ITA) | Be Pink | mt |
| 36 | Monika Brzezna (POL) | | mt |
| 37 | Tetiana Yashchenko (UKR) | | mt |
| 38 | Valeriya Kononenko (UKR) | | mt |
| 39 | Zuzanna Chylinska (POL) | | mt |
| 40 | Maaike Coljé (P-B) | Arkéa Pro Cycling Team | mt |
| 41 | Noemi Lucrezia Eremita (ITA) | Isolmant - Premac - Vittoria | mt |
| 42 | Eliska Kvasnicková (RTC) | | mt |
| 43 | Francesca Baroni (ITA) | | mt |
| 44 | Nela Slaníková (RTC) | | mt |
| 45 | Victoire Joncheray (FRA) | | mt |
| 46 | Matilde Vitillo (ITA) | Be Pink | mt |
| 47 | Anaïs Morichon (FRA) | Arkéa Pro Cycling Team | 0:34 |
| 48 | Lucie Liboreau (FRA) | Arkéa Pro Cycling Team | mt |
| 49 | Lucy Mayrhofer (ALL) | | mt |
| 50 | Lana Eberle (ALL) | Ceratizit - Wnt | mt |
| 51 | Barbora Nemcová (RTC) | | mt |
| 52 | Carmela Cipriani (ITA) | Isolmant - Premac - Vittoria | 0:40 |
| 53 | Gaia Segato (ITA) | | 0:42 |
| 54 | Fabienne Buri (SUI) | | 0:43 |
| 55 | Lara Scarselli (ITA) | Aromitalia - Basso Bikes - Vaiano | 0:45 |
| 56 |  Safia Al Sayegh (EAU) | Uae Team Adq | mt |
| 57 | Imogen Cotter (IRL) | Fenix-Deceuninck Development Team | 0:43 |
| 58 | Nikol Plosaj (POL) | | 0:45 |
| 59 | Giulia Vallotto (ITA) | | mt |
| 60 |  Valantine Nzayisenga (RWA) | | 0:52 |
| 61 | Nicole Hartychová (RTC) | | mt |
| 62 | Ségolène Thomas (FRA) | | mt |
| 63 | Alice Palazzi (ITA) | | 0:55 |
| 64 | Marina Varenyk (UKR) | Eneicat - Cmteam - Seguros Deportivos | mt |
| 65 |  Maayan Tzur (ISR) | Israel Premier Tech Roland | mt |
| 66 |  Emilie Fortin (CAN) | | mt |
| 67 | Yuliia Biriukova (UKR) | | 0:57 |
| 68 | Janine Schneider (ALL) | | 0:59 |
| 69 |  Jennifer Tatiana Ducuara Acevedo (COL) | Massi - Tactic | mt |
| 70 | Beatrice Pozzobon (ITA) | | 1:03 |
| 71 | Emma Redaelli (ITA) | | mt |
| 72 | Wiktoria Pikulik (POL) | | 1:05 |
| 73 | Anne Knijnenburg (P-B) | | mt |
| 74 | Chiara Reghini (ITA) | | 1:09 |
| 75 | Kirstie van Haaften (P-B) | Parkhotel Valkenburg | 1:17 |
| 76 | Nika Myalitsina (RUS) | | 1:20 |
| 77 | Virginia Bortoli (ITA) | | mt |
| 78 | Martina Silvestri (ITA) | | mt |
| 79 |  Claire Windsor (E-U) | | 1:25 |
| 80 | Sara Pepoli (ITA) | Isolmant - Premac - Vittoria | mt |
| 81 | Nofar Maoz (ISR) | Israel Premier Tech Roland | mt |
| 82 | Beatrice Rossato (ITA) | Isolmant - Premac - Vittoria | 1:29 |
| 83 |  Anabel María Fernanda Yapura Plaza (ARG) | | 1:31 |
| 84 | Sara Casasola (ITA) | Born to Win G20 Ambedo | mt |
| 85 | Sara Piffer (ITA) | | 1:34 |
| 86 | Marion Norbert Riberolle (FRA) | Fenix-Deceuninck Development Team | mt |
| 87 | Gemma Sernissi (ITA) | GB Junior Team Piemonte Pedale Castanese A.S.D. | 1:40 |
| 88 | Ashley Frye (E-U) | | mt |
| 89 |  Daniela Schmidsberger (AUT) | | 1:47 |
| 90 | Kamila Pastuszek (POL) | | mt |
| 91 | Iris Gómez Argiles (ESP) | | mt |
| 92 | Patricia Ortega Ruiz (ESP) | Massi - Tactic | 1:58 |
| 93 |  Sara van De Vel (BEL) | Fenix-Deceuninck Development Team | 2:04 |
| 94 | Katharina Becker (ALL) | | mt |
| 95 | Franziska Brauße (ALL) | Ceratizit - Wnt | mt |
| 96 | Annika Schelb (ALL) | | 2:14 |
| 97 | Yelyzaveta Holod (UKR) | | 2:23 |
| 98 | Marta Pavesi (ITA) | | 2:25 |
| 99 | Dorota Przezak (POL) | | 2:28 |
| 100 | Fien Van Eynde (BEL) | Fenix-Deceuninck Development Team | 2:31 |
| 101 | Fabia Maramotti (ITA) | | 2:36 |
| 102 | Giorgia Bariani (ITA) | | 2:38 |
| 103 | Gabriela Bártová (RTC) | | mt |
| 104 | Adar Shriki (ISR) | Israel Premier Tech Roland | mt |
| 105 | Anja Bertleff (ALL) | | 2:52 |
| 106 |  Llori Sharpe (JAM) | | 2:58 |
| 107 | Nikola Bajgerová (RTC) | | 3:01 |
| 108 | Romina Costantini (ITA) | | 3:08 |
| 109 | Anna Hicks (E-U) | | 3:14 |
| 110 | Jadee Treffeisen (ALL) | | 3:16 |
| 111 | Milena Del Sarto (ITA) | Aromitalia - Basso Bikes - Vaiano | 3:18 |
| 112 |  Nesrine Houili (ALG) | | mt |
| 113 | Sonia Rossetti (ITA) | GB Junior Team Piemonte Pedale Castanese A.S.D. | 3:26 |
| 114 | Valeria Curnis (ITA) | | 3:52 |
| 115 | Femke Gerritse (P-B) | Parkhotel Valkenburg | 3:57 |
| 116 | Giulia Miotto (ITA) | GB Junior Team Piemonte Pedale Castanese A.S.D. | 3:59 |
| 117 | Monica Castagna (ITA) | | 4:26 |
| 118 | Beatrice Caudera (ITA) | | 4:43 |
| 119 | Kayla Davis (E-U) | Israel Premier Tech Roland | mt |
| 120 | Elena Contarin (ITA) | | 4:54 |
| 121 | Marta Romance I Oms (ESP) | | 5:26 |
| 122 | Marie Lagershausen (ALL) | | mt |
| 123 | Diane Ingabire (RWA) | | 6:26 |
| 124 | Cara O'Neill (E-U) | | mt |
| 125 | Martina Testa (ITA) | | 6:28 |
| 126 | Alice Capasso (ITA) | | 6:37 |
| 127 | Karoline Goldschmidt (ALL) | | 7:07 |
| 128 | Alina Bogdan (UKR) | | 7:11 |
| 129 | Nataliia Safroniuk (UKR) | | 7:26 |
| 130 | Francesca Tommasi (ITA) | | 7:27 |
| 131 |  Laura Belohvoscika (LAT) | | 7:32 |
| 132 | Katerina Kohoutková (RTC) | | 7:37 |
| 133 | Beatrice Roda (ITA) | | 7:41 |
| 134 | Sara Luccon (ITA) | | 8:01 |
| 135 | Maja Tracka (POL) | | |
| 136 | Alessia Foligno (ITA) | | |
| 137 | Tatiana Chili (ITA) | | |
| 138 | Esther Luna Gazzola (ITA) | | |
| 139 | Giulia Giuliani (ITA) | GB Junior Team Piemonte Pedale Castanese A.S.D. | |
| 140 | Vittoria Ruffilli (ITA) | GB Junior Team Piemonte Pedale Castanese A.S.D. | |
| 141 |  Tess Lawson (G-B) | | |
| 142 | Viktoriia Parkhomiuk (UKR) | | |
| 143 | Anastasiia Ishman (UKR) | | |
| 144 | Lara Röhricht (ALL) | | |
| 145 | Julia van Bokhoven (P-B) | Parkhotel Valkenburg | |
| 146 | Kitija Siltumena (LAT) | | |
| 147 | Lasma Elza Vaivode (LAT) | | |
| 148 | Evelina Ermane-Marcenko (LAT) | | |
