| 1 |  Marcela Elizabeth Prieto Castañeda (MEX) | Swapit Agolico | 11:05:32 |
| 2 |  Flávia Oliveira (BRE) | Health Mate - Cyclelive | 0:05 |
| 3 |  Jazmín Gabriela Soto Lopez (GUA) | | 0:05 |
| 4 |  Leidy Natalia Muñoz Ruiz (COL) | | 0:05 |
| 5 | Jeniffer Alejandra Medellin Rodriguez (COL) | | 0:10 |
| 6 | Luisa Fernanda Motavita Duarte (COL) | | 0:10 |
| 7 | Brenda Andrea Santoyo Perez (MEX) | | 0:10 |
| 8 | Blanca Liliana Moreno Canchon (COL) | Astana Women's | 0:14 |
| 9 | Andrea Ramírez Fregoso (MEX) | Swapit Agolico | 0:14 |
| 10 | Lorena Maria Vargas Villamil (COL) | | 0:19 |
| 11 | Sindra Viviana Narvaez Rodriguez (COL) | | 0:19 |
| 12 | Jeimy Lorena Beltrán Garcia (COL) | | 0:46 |
| 13 | Florida Isabel De León Gonzalez (GUA) | | 1:09 |
| 14 | Anet Barrera Esparza (MEX) | Swapit Agolico | 1:09 |
| 15 |  Marcela Rubiano Garces (CRC) | | 1:42 |
| 16 | Edith Guillen Chavarria (CRC) | | 1:42 |
| 17 | Karol Julieth Moreno Avella (COL) | | 1:45 |
| 18 | Luisa Fernanda Naranjo Henao (COL) | Pedalea - Los Cabos - JB Ropa Deportiva | 1:48 |
| 19 | Maria José Vargas Barrientos (CRC) | Swapit Agolico | 2:40 |
| 20 |  Angie Gómez (HON) | | 2:42 |
| 21 |  Stephanie Nathalie Subercaseaux Vergara (CHI) | | 3:02 |
| 22 | Valentina Blandon Ramirez (COL) | | 4:50 |
| 23 | Laura Sofia Castillo Murillo (COL) | Pedalea - Los Cabos - JB Ropa Deportiva | 5:00 |
| 24 | Yolanda Valderrama Henao (COL) | | 5:01 |
| 25 | Paola Andrea Muñoz Grandon (CHI) | Swapit Agolico | 5:44 |
| 26 |  Donelys Cariño Rivera (PUR) | | 8:50 |
| 27 | Janet González (COL) | | 9:26 |
| 28 | Marcia Fiorella Rojas Hernandez (CRC) | | 9:39 |
| 29 | Jennifer Morales Milanes (CRC) | | 11:16 |
| 30 | Susan Camacho Fallas (CRC) | | 15:29 |
| 31 | Cintia Eugenia Lee Lopez (GUA) | | 15:46 |
| 32 | Marisol Tellado (PUR) | | 16:26 |
| 33 | Julith Fabiola Arroyo Salas (CRC) | | 17:22 |
| 34 | Lady Johana Sepulveda Duran (COL) | | 19:40 |
| 35 | Lizbeth Ureño Armas (MEX) | | 19:50 |
| 36 |  Mary Elizabeth Maroon (E-U) | | 19:56 |
| 37 | Stephany Castillo Malouf (GUA) | | 22:41 |
| 38 | Linda Daniela Menendez (HON) | | 27:58 |
| 39 | Samantha Quijano (E-U) | | 30:50 |
| 40 | Claudia Angelica Roncancio Cabra (COL) | | 32:02 |
| 41 | Cristine De Mezerville Ferreto (CRC) | | 35:46 |
| 42 | Diana Solano Rojas (CRC) | | 39:58 |
| 43 | Priscilla Chevez Gonzalez (CRC) | | 49:59 |
| 44 | Daniela Irais Gonzalez Garcia (MEX) | | 50:11 |
| 45 | Susana Patricia Santos Santos (GUA) | | 55:44 |
| 46 | Kennia Damaris Vargas Umaña (CRC) | | 56:44 |
| 47 | Karen Elisa Amaya (HON) | | 59:19 |
| 48 | Leydy Xiomara Buitrago Perdomo (COL) | | 1:06:10 |
| 49 | Deborah Vega Gómez (PUR) | | 1:16:48 |
| 50 | Karen Canales (HON) | | 1:17:16 |
| 51 | Laura Lopez Torres (CRC) | | 1:19:28 |
| 52 | María Morales (GUA) | | 1:20:20 |
| 53 | Cristina Torres Santini (PUR) | | 1:25:48 |
| 54 | Maria Andre Girón de León (GUA) | | 1:28:14 |
| 55 | Yadira Torres Ruiz (PUR) | | 1:29:32 |
| 56 | Casandra Surillo (PUR) | | 1:54:03 |
