| 1 |  Paul Double (G-B) | Team Jayco Alula | 4:18:23 |
| 2 |  Jarno Widar (BEL) | Lotto Development Team | mt |
| 3 |  Diego Ulissi (ITA) | Xds Astana Team | mt |
| 4 | Ben Tulett (G-B) | Team Visma | Lease A Bike | mt |
| 5 |  Magnus Sheffield (E-U) | | 0:03 |
| 6 | Davide De Pretto (ITA) | Team Jayco Alula | mt |
| 7 |  Alexey Lutsenko (KAZ) | Israel - Premier Tech | 0:05 |
| 8 | Alessandro Covi (ITA) | Uae Team Emirates - Xrg | mt |
| 9 | Simone Velasco (ITA) | Xds Astana Team | mt |
| 10 |  Brandon Smith Rivera Vargas (COL) | | mt |
| 11 | Mark Donovan (G-B) | Q36.5 Pro Cycling Team | mt |
| 12 |  Rafal Majka (POL) | Uae Team Emirates - Xrg | mt |
| 13 | Alessandro Pinarello (ITA) | VF Group - Bardiani CSF - Faizane | 0:08 |
| 14 |  Milan Vader (P-B) | Q36.5 Pro Cycling Team | 0:10 |
| 15 | Mauri Vansevenant (BEL) | Soudal - Quick Step | mt |
| 16 |  Mattéo Vercher (FRA) | Totalenergies | mt |
| 17 | Lorenzo Galimberti (ITA) | | mt |
| 18 |  Igor Arrieta Lizarraga (ESP) | Uae Team Emirates - Xrg | 0:13 |
| 19 | Giovanni Carboni (ITA) | Unibet Tietema Rockets | mt |
| 20 |  Emil Herzog (ALL) | Red Bull - Bora - Hansgrohe | 0:17 |
| 21 |  Andrii Ponomar (UKR) | | 0:27 |
| 22 | Johan Meens (BEL) | Wagner Bazin Wb | 0:28 |
| 23 | Viktor Soenens (BEL) | Soudal - Quick-Step Devo Team | mt |
| 24 | Luca Cretti (ITA) | | 0:32 |
| 25 | Markel Beloki (ESP) | EF Education - Easypost | 0:33 |
| 26 | Cesare Chesini (ITA) | | mt |
| 27 |  Jefferson Alexander Cepeda Ortiz (EQU) | EF Education - Easypost | mt |
| 28 |  Matteo Badilatti (SUI) | Q36.5 Pro Cycling Team | mt |
| 29 |  Jørgen Nordhagen (NOR) | Team Visma | Lease A Bike | mt |
| 30 | Mattia Bais (ITA) | Team Polti Visitmalta | mt |
| 31 | Manuele Tarozzi (ITA) | VF Group - Bardiani CSF - Faizane | mt |
| 32 |  Pavel Novák (RTC) | | mt |
| 33 | Edison Alejandro Callejas Santos (COL) | | 0:39 |
| 34 | Felix Engelhardt (ALL) | Team Jayco Alula | 0:44 |
| 35 | Alessandro Tonelli (ITA) | Team Polti Visitmalta | mt |
| 36 |  Dennis Lock (DAN) | | mt |
| 37 | Colin Stüssi (SUI) | | 0:46 |
| 38 | Tijmen Graat (P-B) | Team Visma | Lease A Bike | 0:48 |
| 39 | Odd Christian Eiking (NOR) | Unibet Tietema Rockets | 0:51 |
| 40 | Lennart Jasch (ALL) | | mt |
| 41 | Luca Paletti (ITA) | VF Group - Bardiani CSF - Faizane | mt |
| 42 | Alex Tolio (ITA) | VF Group - Bardiani CSF - Faizane | mt |
| 43 | Ian López de San Roman (E-U) | | 1:05 |
| 44 |  Edgar David Cadena Martinez (MEX) | | 1:11 |
| 45 |  Nahom Zeray (ERI) | | 1:16 |
| 46 |  Roberto Carlos González Castillero (PAN) | Team Solution Tech - Vini Fantini | 1:18 |
| 47 | Leander Van Hautegem (BEL) | Wagner Bazin Wb | 1:21 |
| 48 | Kirilo Tsarenko (UKR) | Team Solution Tech - Vini Fantini | 1:25 |
| 49 | Sergio Meris (FRA) | Unibet Tietema Rockets | mt |
| 50 | Florian Samuel Kajamini (ITA) | Xds Astana Team | mt |
| 51 |  Luke Tuckwell (AUS) | | 1:30 |
| 52 | Kevin Biehl (DAN) | | 1:41 |
| 53 | Alessandro Fancellu (ITA) | | 1:47 |
| 54 | Giacomo Villa (ITA) | Wagner Bazin Wb | mt |
| 55 | Marco Martini (ITA) | | 1:52 |
| 56 |  Vicente Rojas Naranjo (CHI) | VF Group - Bardiani CSF - Faizane | 2:04 |
| 57 | Thomas Gloag (G-B) | Team Visma | Lease A Bike | 2:14 |
| 58 | Pierre-Henry Basset (FRA) | | mt |
| 59 | Pierre Latour (FRA) | Totalenergies | mt |
| 60 |  Alan Hatherly (AFS) | Team Jayco Alula | 2:57 |
| 61 | Ben Swift (G-B) | | mt |
| 62 | Jan Hirt (RTC) | Israel - Premier Tech | mt |
| 63 | Fabien Doubey (FRA) | Totalenergies | mt |
| 64 | German Dario Gomez Becerra (COL) | Team Polti Visitmalta | 3:13 |
| 65 | Baptiste Huyet (FRA) | Unibet Tietema Rockets | 3:16 |
| 66 | Enea Sambinello (ITA) | | 3:25 |
| 67 | Alessio Martinelli (ITA) | VF Group - Bardiani CSF - Faizane | mt |
| 68 | Davide Baldaccini (ITA) | Team Solution Tech - Vini Fantini | mt |
| 69 | Davide De Cassan (ITA) | Team Polti Visitmalta | 3:27 |
| 70 | Kamiel Eeman (BEL) | Lotto Development Team | 3:51 |
| 71 | Adam Toupalík (RTC) | Unibet Tietema Rockets | mt |
| 72 | Lorenzo Finn (ITA) | | 3:55 |
| 73 | Tim Rex (BEL) | Team Visma / Lease a Bike Development | 4:13 |
| 74 | Adrien Maire (FRA) | Unibet Tietema Rockets | 4:53 |
| 75 |  Sebastian Putz (AUT) | | mt |
| 76 | Alberto Bettiol (ITA) | Xds Astana Team | 5:56 |
| 77 | Marco Tizza (ITA) | | mt |
| 78 | Colby Simmons (E-U) | | 6:15 |
| 79 | Andrew August (E-U) | | mt |
| 80 | Kim Alexander Heiduk (ALL) | | 6:16 |
| 81 | Jay Vine (AUS) | Uae Team Emirates - Xrg | 6:21 |
| 82 | Alistair MacKellar (AUS) | EF Education - Easypost | mt |
| 83 | Floris De Tier (BEL) | Wagner Bazin Wb | mt |
| 84 | Thomas Pesenti (ITA) | Soudal - Quick-Step Devo Team | mt |
| 85 | Adrien Boichis (FRA) | | 6:43 |
| 86 | Diego Bracalente (ITA) | | 6:54 |
| 87 | Alex Baudin (FRA) | EF Education - Easypost | 7:15 |
| 88 | Alexander Konychev (ITA) | | 8:59 |
| 89 | Pietro Mattio (ITA) | Team Visma / Lease a Bike Development | mt |
| 90 | Domenico Cirlincione (ITA) | | mt |
| 91 | Pablo Garcia Frances (ESP) | Team Polti Visitmalta | mt |
| 92 | Federico Guzzo (ITA) | | mt |
| 93 | William Smith (G-B) | Team Visma / Lease a Bike Development | mt |
| 94 | Marcel Camprubi Pijuan (ESP) | Q36.5 Pro Cycling Team | 10:01 |
| 95 | Pascal Eenkhoorn (P-B) | Soudal - Quick Step | 10:04 |
| 96 | Mark Stewart (G-B) | Team Solution Tech - Vini Fantini | mt |
| 97 | Cedrik Bakke Christophersen (NOR) | | 10:10 |
| 98 |  Emils Liepins (LAT) | Q36.5 Pro Cycling Team | 10:25 |
| 99 | Walter Calzoni (ITA) | Q36.5 Pro Cycling Team | mt |
| 100 | Andrea Piras (ITA) | Team Solution Tech - Vini Fantini | mt |
| 101 | Niels Driesen (BEL) | Lotto Development Team | 13:15 |
| 102 | Arne Baers (BEL) | Lotto Development Team | 13:16 |
| 103 |  Emry Faingezicht (ISR) | | mt |
| 104 | Milan Donie (BEL) | Lotto Development Team | mt |
| 105 |  Luke Valenti (CAN) | | mt |
| 106 | Kristian Sbaragli (ITA) | Team Solution Tech - Vini Fantini | mt |
| 107 | Matteo Zurlo (ITA) | | mt |
| 108 |  Nariyuki Masuda (JPN) | | mt |
| 109 |  Negasi Haylu Abreha (ETH) | | mt |
| 110 | Alessandro Cattani (ITA) | | mt |
| 111 | Federico Savino (ITA) | Soudal - Quick-Step Devo Team | mt |
| 112 |  Adam Rafferty (IRL) | | mt |
| 113 | Luca Bagnara (ITA) | | mt |
| 114 | Janis Peter (ALL) | | mt |
| 115 | Moritz Kretschy (ALL) | | mt |
| 116 | Brady Gilmore (AUS) | | mt |
| 117 |  Ivo Emanuel Alves Oliveira (POR) | Uae Team Emirates - Xrg | mt |
| 118 |  Campbell Stewart (NZL) | Team Jayco Alula | mt |
| 119 | Baptiste Vadic (FRA) | Totalenergies | mt |
| 120 | Mathieu Burgaudeau (FRA) | | 13:21 |
| 121 | Milan Menten (BEL) | Lotto | mt |
| 122 | Martin Marcellusi (ITA) | VF Group - Bardiani CSF - Faizane | mt |
| 123 | Samuele Privitera (ITA) | | 16:00 |
| 124 | Andrea Raccagni Noviero (ITA) | Soudal - Quick Step | mt |
| 125 | Gabriele Raccagni (ITA) | Team Polti Visitmalta | mt |
| 126 | Mattia Negrente (ITA) | | mt |
| 127 | Manuel Oioli (ITA) | | mt |
| 128 | Davide Donati (ITA) | | 16:03 |
| 129 | Ugo Fabries (FRA) | | mt |
| 130 | Valentin Retailleau (FRA) | Totalenergies | mt |
| 131 | Giovanni Bortoluzzi (ITA) | | mt |
| 132 | Marco Palomba (ITA) | | mt |
| 133 | Filippo d'Aiuto (ITA) | | mt |
| 134 | Lukas Meiler (ALL) | | mt |
| 135 | Marc Cabedo Hernandez (ESP) | | 16:10 |
| 136 | Lorenzo Nespoli (ITA) | | 18:03 |
| 137 | Carlos Ariel Samudio Carrera (PAN) | Team Solution Tech - Vini Fantini | 19:41 |
| 138 | Marino Kobayashi (JPN) | | 21:22 |
| 139 | Davide Persico (ITA) | Wagner Bazin Wb | 21:23 |
| 140 | Aldo Taillieu (BEL) | Lotto Development Team | mt |
| 141 | Alessio Delle Vedove (ITA) | | mt |
| 142 | Riccardo Archetti (ITA) | | mt |
| 143 | Rosola Kevin Pezzo (ITA) | | mt |
| 144 | Cristian Remelli (ITA) | | mt |
| 145 | Daniel Geismayr (AUT) | | mt |
| 146 | Oded Kogut (ISR) | Israel - Premier Tech | mt |
| 147 | Koki Kamada (JPN) | | mt |
| 148 | Luca Martignago (ITA) | | mt |
| 149 | Jose Antonio Prieto De Luna (MEX) | | mt |
| 150 | Alessio Menghini (ITA) | | 21:28 |
| 151 | Samuele Bonetto (ITA) | | mt |
| 152 | Manuel Penalver Aniorte (ESP) | Team Polti Visitmalta | mt |
| 153 | Antonio Bonaldo (ITA) | | mt |
| 154 | Yeferson Camargo (COL) | | mt |
| 155 | Marco Fermanelli (ITA) | | mt |
| 156 |  Davide Basso (HON) | | 21:32 |
| 157 | Stefano Baffi (ITA) | | mt |
| 158 | Emanuel Zangerle (AUT) | | 21:39 |
| 159 | Manabu Ishibashi (JPN) | | mt |
| 160 | Caleb Ewan (AUS) | | 21:59 |
| 161 | Louka Matthys (BEL) | Wagner Bazin Wb | 22:21 |
| 162 | Sebastiano Minoia (ITA) | | 22:24 |
| 163 | Artem Shmidt (E-U) | | 22:50 |
| 164 | Thomas Turri (ITA) | | 24:15 |
| 165 | Dominik Amann (AUT) | | 24:20 |
| 166 | Luca Collinelli (ITA) | | 24:51 |
| 167 | Samuele Mion (ITA) | | 25:23 |
| 168 | Ivan Toselli (ITA) | | 25:31 |
| 169 | Jason Tesson (FRA) | Totalenergies | |
| 170 | Samuel Quaranta (ITA) | | |
| 171 | Christian Danilo Pase (ITA) | | |
| 172 | Gauthier Servranckx (BEL) | Soudal - Quick-Step Devo Team | |
| OTL | Samuele Scappini (ITA) | | - |
